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DIRECT PROBE TECHNIQUE AND AMPLIFIED PROBE TECHNIQUE

Apurva Posted Wed 23rd of October, 2019 04:40:57 AM
WHAT IS THE MAJOR DIFFERENCE IN A DIRECT PROBE TECHNIQUE AND AN AMPLIFIED PROBE TECHNIQUE
SuperCoder Answered Thu 24th of October, 2019 06:53:49 AM

Hi, 

 

In ‘direct probe technique’, the lab analyst uses direct nucleic acid probes which reacts directly with nucleic acids in the test sample and identify specific DNA or RNA sequences that help identify the isolated organism as a specific type. If the test sample contains the organism of interest, the reaction (e.g., hybridization) of these elements will create a detectable endpoint.

In ‘amplified probe technique’, the lab analyst uses amplification method to identify specific DNA or RNA sequences that help identify the isolated organism as a specific type. In amplification technique, the lab analyst makes more copies of desired gene for study by processes such as polymerase chain reaction, called PCR, or transcription of DNA to RNA and reverse transcription from RNA to make an additional copy of the DNA.

 

 

Thanks

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